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EE

EE (Edwards and Ewing) Broth Mossel is a selective enrichment medium used for the isolation and enumeration of Enterobacteriaceae in food and environmental samples. The brand is associated with standardized microbiology media formulations, particularly those referenced in ISO 21528-1 and ISO 21528-2 methods for detection and enumeration of Enterobacteriaceae. EE Broth Mossel contains bile salts and brilliant green as selective agents, which inhibit Gram-positive bacteria and many Gram-negative non-Enterobacteriaceae, while allowing growth of Enterobacteriaceae. The medium is supplied in multiple formats to accommodate different laboratory workflows: as a ready-to-use solid medium in 90 mL and 100 mL volumes (SKUs 45H326 and 45H327) with a 180-day shelf life, and as a powder in 500 mL and 10 L sizes (SKUs 39M727 and 39M725) with a 2-year shelf life. The powder form is packaged in bottles and requires reconstitution with distilled water and sterilization before use. The solid form is pre-poured in tubes or bottles, ready for direct inoculation. 3G Electric stocks these EE Broth Mossel products to support quality control laboratories in food processing, pharmaceutical manufacturing, and water testing facilities. The medium is formulated to meet the specifications of standard methods, ensuring consistent performance for most probable number (MPN) determinations and presence/absence tests. 3G Electric provides datasheets with detailed composition, preparation instructions, and quality control parameters for each SKU. Logistics support includes temperature-controlled storage recommendations and batch traceability. Procurement specialists can request quotes for single SKUs or bulk orders, with global shipping from 3G Electric's Singapore hub. The product range covers both ready-to-use and dehydrated formats, allowing laboratories to choose based on throughput, storage capacity, and preparation preferences. EE Broth Mossel is a standard medium in microbiological testing for Enterobacteriaceae, and 3G Electric's inventory ensures availability for routine testing and method compliance.

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About EE

EE (Edwards and Ewing) Broth Mossel is a selective enrichment medium used for the isolation and enumeration of Enterobacteriaceae in food and environmental samples. The brand is associated with standardized microbiology media formulations, particularly those referenced in ISO 21528-1 and ISO 21528-2 methods for detection and enumeration of Enterobacteriaceae. EE Broth Mossel contains bile salts and brilliant green as selective agents, which inhibit Gram-positive bacteria and many Gram-negative non-Enterobacteriaceae, while allowing growth of Enterobacteriaceae. The medium is supplied in multiple formats to accommodate different laboratory workflows: as a ready-to-use solid medium in 90 mL and 100 mL volumes (SKUs 45H326 and 45H327) with a 180-day shelf life, and as a powder in 500 mL and 10 L sizes (SKUs 39M727 and 39M725) with a 2-year shelf life. The powder form is packaged in bottles and requires reconstitution with distilled water and sterilization before use. The solid form is pre-poured in tubes or bottles, ready for direct inoculation. 3G Electric stocks these EE Broth Mossel products to support quality control laboratories in food processing, pharmaceutical manufacturing, and water testing facilities. The medium is formulated to meet the specifications of standard methods, ensuring consistent performance for most probable number (MPN) determinations and presence/absence tests. 3G Electric provides datasheets with detailed composition, preparation instructions, and quality control parameters for each SKU. Logistics support includes temperature-controlled storage recommendations and batch traceability. Procurement specialists can request quotes for single SKUs or bulk orders, with global shipping from 3G Electric's Singapore hub. The product range covers both ready-to-use and dehydrated formats, allowing laboratories to choose based on throughput, storage capacity, and preparation preferences. EE Broth Mossel is a standard medium in microbiological testing for Enterobacteriaceae, and 3G Electric's inventory ensures availability for routine testing and method compliance.

Common Applications

  • Food processing quality control — EE Broth Mossel (solid, 90 mL) for MPN enumeration of Enterobacteriaceae in raw meat and dairy products.
  • Pharmaceutical raw material testing — EE Broth Mossel (powder, 500 mL) for selective enrichment of Enterobacteriaceae in excipients and active ingredients.
  • Water and wastewater analysis — EE Broth Mossel (solid, 100 mL) for presence/absence testing of Enterobacteriaceae in potable and surface water.
  • Environmental surface monitoring — EE Broth Mossel (powder, 10 L) for bulk preparation of medium used in swab and rinse tests in cleanrooms.
  • Pet food manufacturing — EE Broth Mossel (solid, 90 mL) for routine screening of Enterobacteriaceae in dry and wet pet food formulations.
  • Beverage production — EE Broth Mossel (powder, 500 mL) for detection of Enterobacteriaceae in fruit juices and soft drinks during HACCP verification.

Frequently asked questions

Common questions about EE from 3G Electric.

What is the difference between the solid and powder forms of EE Broth Mossel supplied by 3G Electric?

The solid form (SKUs 45H326 and 45H327) is a ready-to-use medium in 90 mL or 100 mL volumes, pre-sterilized and packaged in tubes or bottles. It has a 180-day shelf life and requires no preparation—just inoculate directly. The powder form (SKUs 39M725 and 39M727) is a dehydrated medium in 500 mL or 10 L sizes, packaged in bottles with a 2-year shelf life. It must be reconstituted with distilled water, heated to dissolve, and sterilized by autoclaving before use. The powder form is more economical for high-volume labs and allows customization of batch sizes.

What is the shelf life of EE Broth Mossel products from 3G Electric?

The solid EE Broth Mossel (SKUs 45H326 and 45H327) has a shelf life of 180 days from the date of manufacture. The powder form (SKUs 39M725 and 39M727) has a shelf life of 2 years. Both should be stored according to the manufacturer's recommendations—typically at 2–8°C for the solid form and at room temperature (15–30°C) for the powder form, away from moisture and direct sunlight. Always check the expiration date on the label before use.

Which standard methods are EE Broth Mossel products from 3G Electric suitable for?

EE Broth Mossel is formulated for the selective enrichment of Enterobacteriaceae and is commonly used in methods such as ISO 21528-1 (detection) and ISO 21528-2 (enumeration by MPN). It is also referenced in other food and water microbiology standards. The medium's selective agents (bile salts and brilliant green) inhibit non-target organisms while allowing Enterobacteriaceae to grow. For specific method compliance, refer to the datasheet provided by 3G Electric for each SKU.

Can I order EE Broth Mossel in bulk from 3G Electric?

Yes, 3G Electric supports bulk orders for EE Broth Mossel products. The powder form is available in 10 L bottles (SKU 39M725), which is suitable for high-volume testing. For larger quantities, contact 3G Electric for a quote. The solid form is typically ordered per unit, but volume discounts may apply for multiple cases. 3G Electric provides logistics support for global shipping, including temperature-controlled transport for the solid form if required.

What is the MPN method and how does EE Broth Mossel support it?

The Most Probable Number (MPN) method is a statistical technique used to estimate the concentration of viable microorganisms in a sample. EE Broth Mossel is used as the enrichment medium in MPN tests for Enterobacteriaceae. The solid form (90 mL or 100 mL) is inoculated with sample dilutions and incubated. Growth is indicated by turbidity or gas production. The number of positive tubes is then used to calculate the MPN per gram or milliliter. 3G Electric's EE Broth Mossel is formulated to meet the requirements of MPN procedures in food and water testing.

Does 3G Electric supply EE products?

Yes — 3G Electric (S) Pte. Ltd. supplies EE products. We stock the EE catalog and supply genuine factory-original products with full manufacturer warranty and technical support.